Hasta Başı-Yerinde Değerlendirme /On Site-Rapid On Site Evaluation (ROSE)

Patient fast on-site evaluation (rose), during a biopsy procedure is an assessment of the adequacy of the patient's diagnostic sample.

The procedure itself is done in order to inform and optimize the subsequent patient management.

Key features

Fast-patient-in-place evaluation (on-site), have sufficient training and sent to pathology intern, sitoteknolog can be performed by clinical or physician.
Fast patient-one of the main objectives of assessment in place, triage, including the amount of material obtained for diagnostic biopsy during the procedure, it is going to be any additional work (e.g., flow cytometry, molecular tests, etc.) foreseen, in order to optimize your.
Fast-patient samples in place evaluation of fine-needle aspiration (Fna) smears of aspirates or biopsy taken from a core biopsy from the tap, slide consists of a touch.
ROSE examined the diagnostic biopsy is performed for the transition statements.
Depending on the context, on-site diagnosis; short and categorical (i.e. sufficient or non-diagnostic) or detailed and specific (i.e. supporting squamous cell carcinoma) may be.
The benefits of Rose: the number of biopsy passes, procedure number, and reduces the cost of needed to reach a definitive diagnosis.

Rapid patient assessment (on-site) application fields of

Fast-patient evaluation in place, cytological biopsy procedures can be performed during any anatomical region
Fast-patient assessment in place, and purpose of the relevant organization, anatomical region, hypothetical diagnosis, biopsy, pathologists may be connected to the operator and

Laboratory

ROSE, the performance of the procedure in general terms biopsy can be optimized so as to provide benefit to the patient 3:

Biopsy during the procedure necessary to obtain a diagnosis and the number of transitions that are made reducing the number of general procedures that are treatable ( Cancer Cytopathol 2013;121:518 ).
Next cellular materials and ancillary diagnostic tests for the efficient disposal of inform, prescribed diagnostic immunohistochemical studies and flow cytometry, and molecular testing, including obtaining sufficient material necessary for therapeutic ( Sitopatol Cancer 2013;121:544 )
Rose's performance, availability of equipment, laboratory personnel, and procedural depending on a number of factors including the frequency, can reduce the cost of getting a diagnosis ( PLOS ONE 2015;10:e0135466 )

The benefits of Rose may be limited in some cases:

Usually only a small volume cysts cyst fluid can be analyzed, and this is my rose ( Cibas: Cytology Diagnostic principles and Clinical correlates, 5. Print, 2021 ) eliminates the benefits of.
Needle core biopsy of pancreatic solid lesions sampled by the lack of enough cells in a sample of prepart touch( Surg Endosc 2017;31:225 )
Inappropriate touch preparation technique, due to the depletion of the material diagnostic core needle biopsy ( J Am Soc Cytopathol 2020;9:322 )

ROSE pathology triggered by the help of studies may include the following:

Molecular testing ( Curr Christoph F 2017;24:103 , Acta Cytol 2016;60:372 )
Microbiological cultures ( Cancer Cytopathol 2018;126:643 , Cancer Cytopathol 2015;123:612 )
Flow cytometry ( Acta Cytol 2016;60:302 )

No staining should be done according to standard protocol to perform laboratory standards rose.

Diff-Quik Romanowsky dyes are usually used for the Rose slides ( CAP Today: Sitopatoloji ve Daha Fazlası | FNA Sitolojisi – Hızlı Yerinde Değerlendirme – Uygulama Nasıl Değişir [Erişim 20 Aralık 2021] )
Fast or ultrafast Papanicolaou, toluidine blue, fast hematoksil and eosin dyes, available in blue or bright krezil ( J Cytol 2012;29:241 , J Int Med Res 2019;47:626 , Diagn Cytopathol 2019;47:469 )

ROSE, after verification can be accomplished through telepatoloji ( Diagn Cytopathol 2019;47:206 , Cytopathology Was 2020;31:402 )

ROSE equipment required for the procedure:

Sufficient workspace
Disposable pad work (eg. Chux pads)
Microscope
Power supply for microscope
Stain line
Coplin jar
Pen and pencil sharpener
Blank slides
Slide box
RPMI tubes and holders
Formalin jars
Needles to manipulate the tissue
Steel needle holder
Needle disposal box
Biohazard trash
Cleaning supplies
Secure storage
Note 1:
- Equipment can be stored in the operation room or can be made by the pathologist.
- All equipment, storage area, regardless of accreditation must be maintained in accordance with the protocol.

Staff:

ROSE can be provided by a single pathologist
An assistant, a pathologist can be very useful because you can take to prepare slides renditions

Radiology;

ROSE, the biopsy procedure that are included in any radiological methods (USG, CT, etc.) can be performed in conjunction

Molecular / cytogenetic description

Next generation sequencing (NGS) and microRNA classifier reviews, including molecular tests, hucresellig sufficient diagnostic material, with exposed painted can be performed on smears of aspirates.

( Diagn Cytopathol 2019;47:469 , Arch Pathol Lab Med 2018;142:291 )